Acuteband Prestained Protein Ladder
The AcuteBand Pre-stained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a range of molecular weights from 6.5 to 270 kDa. It is designed for monitoring protein separation during SDS-PAGE and verifying Western transfer eciency on membranes and for approximating the size of proteins.
- Range: 6.5 kDa to 270 kDa
- Size: 2 x 250 ul, sufficient for 100-200 lanes
The AcuteBand Pre-stained Protein Ladder is also ideal for Western Blotting by the LICOR System. All markers are bound by the secondary antibody and the bands are clearly visible. No need to mark the bands with a pencil, no need for any additional treatment before the secondary antibody!
Do You Have Data Comparison For Protein Molecular Weights Precision With Other Protein Markers
Yes. Usually, pre-stained marker is written on estimated molecular weight for caution. It is known that the analysis of protein size by an SDS-PAGE is only for estimation because of the intrinsic variation of amino acid composition in all proteins including stained and non-stained ones. For example, a protein which is highly hydrophilic might show a particular higher position in the SDS-PAGE analysis when compared to a hydrophobic one. We did compare the migration patterns of SMOBIOs Protein Markers with other brands, and we concluded that it was difficult to define precision due to the reasons mentioned above. Therefore, in the product description, we suggest our users to calibrate the MW against their interested proteins. Although it is impossible to define “precision” for molecular weight of proteins in SDS-PAGE, we did compare the migration pattern of pre-stained markers with unstained protein marker for calibration. It is concluded that the estimated molecular weight of SMOBIOs pre-stained marker shows a curve matching well with that of unstained native proteins , representing a good estimation of the MW of each pre-stained protein in the SDS-PAGE analysis.
Protein Marker Retention Period: Mentioned
Yes, we have tested our PM2700. The results showed that the PM2700 is stable at -20 for at least two years. It has also shown strong performance for more than 36 months under our careful storage. However, we must only suggest a 2 year retention period for the following reasons: There may be a variation in the environment in storage, and improper use may lead to accumulated damage to the proteins and therefore reduce its retention period.
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Yesblot Western Marker I
- Range: 10 kDa to 200 kDa
- Size: 2 x 250 ul, sufficient for 100 lanes
YesBlot Western Marker I is a ready-to-use mixture with ten IgG-binding proteins covering a wide range of molecular weights from 15 to 200 kDa in TrisGlycine buffer. It contains 4 pre-stained proteins for monitoring protein separation during SDS-PAGE, verification of Western transfer efficiency on membranes and for approximating the protein size. Second, ten IgGbinding proteins can be immuno-detected on film or by CCD imaging. YesBlot Western Marker I is compatible with chemiluminescent, fluorescent, chromogenic or other detection systems.
Why Are There Contrasting Results In Molecular Weights After Using Different Brands Of Protein Markers
A.Different proteins even with similar molecular weights would exhibit apparent disparity from the resulting SDS PAGE due to the difference in the composition of the proteins amino acids . The reason for the disparity is due to the amino acids composition that affects the binding of the protein and SDS. Therefore, we can say that protein marker is a handy tool to estimate molecular weight, but there is no absolute molecular weight standard.
B.While running SDS-PAGE, protein mobility can be affected by the composition of the buffer used, gel percentage, the voltage used, running time, as well as if there is a pre-run.
C.Another recommendation for high molecular weight proteins is to prolong the running time to clarify the relative location of bands.
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How To Choose The Right Protein Ladder
Using a prestained protein ladder when running a western blot helps when you need to:- monitor protein separation during SDS-polyacrylamide gel electrophoresis- verify transfer efficiency between gel and membrane – calculate the approximate size of your protein by overlaying an image of the membrane with the ladder with the image generated by antibody staining.
Using an unstained protein ladder is very useful when you need to accurately calculate the size of your protein. However, an unstained protein ladder can only be visualized following staining with Coomassie or a similar non-specific protein stain. Unstained protein ladders are more accurate for sizing proteins, as the dyes used in prestained ladders can slightly distort the apparent size of the protein ladder proteins on the gel.
Protein ladders are most convenient to use when they are supplied ready to use, with no heating, dilution, or addition of reducing agent required before loading onto the gel. Prestained ladders also can use highlight bands of a different color, to make it easier to identify different size bands.
When choosing the right pre-stained protein ladder, the most important choice is based on the size of your protein of interest. For most researchers, we generally recommend:a) ab116027 for proteins between 10 and 180 kDab) ab116028 for larger proteins up to 245 kDac) ab116029 for smaller proteins down to 3.5 kDad) ab116029 or ab234592 for studying smaller and larger proteins at the same time.
Licor Chameleon Duo Pre
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Will Smobios Protein Markers/ladder Be Washed Out During Western Blotting Process
SMOBIOs Protein Markers/Ladder will be only slightly washed out during Western blotting process. However, the excess of Tween-20 in washing buffer will affect SMOBIOs Protein Markers/Ladder on the transfer membrane.
Here are suggestions for Western blotting process:1. Transfer SMOBIOs Protein Markers/Ladder to membrane with transfer buffer containing 20% methanol to fix SMOBIOs Protein Markers/Ladder on membrane. 2. Wash membrane with PBS or TBS containing less than 0.1% Tween-20.
Will Smobios Protein Markers/ladder Be Affected By The Stripping/deprobing Process With The Presence Of
In normal circumstances, the presence of ME during the stripping/deprobing process will only slightly affect SMOBIOs Protein Markers/Ladder. However, the presence of Tween-20 on PVDF membrane during the stripping/deprobing process has adverse effects on SMOBIOs Protein Markers/Ladder.
Here are suggestions for Western stripping/deprobing process:
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